“To study modulation peculiarities under stress in the systems of internal organs tissue protection from free-radical damage in rats at different stages of puberty”
(research supervisor – Doctor of Medical Sciences, professor V.V. Davydov, 2008−2010 yrs)
Fulfilment of the research project made it possible to establish that a short-term immobilization of rats in their early puberty is not accompanied by some changes in the content of carbonilated proteins and TBK-reactive substances in the brain mitochondrial and microsomal fractions.
Object of study: 300 rats of Wistar line at different ages (1,5 months, 2 months and 12 months). Tissues – blood, brain, liver, heart.
Objective: to analyze the intensity of free radical processes in tissues of internal organs and state of the enzyme systems of their protection against free radical damage in rats at different stages of sexual development under the influence of stressors of varying intensity.
Methods: biochemical, statistic.
Theoretical and practical results: it has been determined that on the stage of sexual maturity in heart, liver and brain of rats, due to changes in activity of analyzed enzymes (catalase, superoxide dismutase, glutathione peroxidase), the conditions were formed for the restriction of functioning of first line antioxidant defense enzyme systems and utilization of endogenous aldehydes: at early puberty age – in brain and liver, and at late puberty age – in heart. It enhances the sensitivity of the animals to the stressor damage under long immobilization.
Novelty: the results give a theoretical substantiation for developing a new perspective direction in increasing the stability of tissues and organs to the stressor damage at the stage of puberty, associated with stimulation of enzyme induction of synthesis of catabolism aldehydes.
Effectiveness: The results have been applied in the course of biochemistry at medicine department of Kharkiv National University named after V.N. Karazin and Kharkiv National Medical University.
Fields of application: medicine and biology.
